Remoción de hidrocarburos en un suelo contaminado, empleando hongos y residuos agroindustriales a través de un proceso de compostaje
Palabras clave:
Hidrocarburos del petróleo, Aceite de motor, Papaya, degradación
Resumen
La contaminación debido a los hidrocarburos del petróleo es un problema grave, el aceite de motor usado es un derivado del petróleo que debido a su inadecuada gestión ocasiona problemas en el suelo. En el presente trabajo se emplearon cinco cepas fúngicas inmovilizadas sobre cáscara de papaya y mezcladas con suelo contaminado con aceite de motor usado, para remover los hidrocarburos totales del petróleo (HTP) en un proceso de compostaje. Las cinco cepas fúngicas fueron capaces de crecer en presencia de petróleo crudo en el proceso de adaptación. La determinación del consumo de hidrocarburos se realizó por medio de gravimetría, se determinó el cambio de humedad y de pH. Durante la evaluación de la remoción de los HTP en el suelo las cepas mostraron porcentajes de degradación entre el 10.85% y 60.83%, la cepa BL1 fue capaz de remover 10.45 g HTP/kg suelo, con una velocidad de 3.39 g HTP/kg suelo×día. Se observó una disminución del pH y la humedad durante el proceso de remoción, como resultado de la acción microbiana. La remoción de HTP presente en el suelo contaminado fue posible por medio del proceso de compostaje mediante la adición de hongos inmovilizados sobre cáscara de papaya. Adicionalmente, el uso de los residuos agroindustriales en este proceso puede ser una forma de revalorización, gestión y disposición final de residuos orgánicos.
Citas
Acevedo-Sandoval, O., Gutierrez-Alcantara, E., Perez-Balan, R., Rodriguez-Vazquez, G., Zamorategui-Molina, A., & Tirado-Torres, D. (2018). Degradation of polycyclic aromatic hydrocarbons using bacterial isolate from the contaminated soil and white rot fungus Pleurotus ostreatus. Appl. Ecol. Environ. Res, 16(4), 3815-3829.
Ahmad, R., Jilani, G., Arshad, M., Zahir, Z. A., & Khalid, A. (2007). Bio-conversion of organic wastes for their recycling in agriculture: an overview of perspectives and prospects. Annals of Microbiology, 57(4), 471-479. https://doi.org/10.1007/BF03175343
Al-Hawash, A. B., Zhang, J., Li, S., Liu, J., Ghalib, H. B., Zhang, X., & Ma, F. (2018). Biodegradation of n-hexadecane by Aspergillus sp. RFC-1 and its mechanism. Ecotoxicology and Environmental Safety, 164, 398-408.
Ambaye, T. G., Chebbi, A., Formicola, F., Prasad, S., Gomez, F. H., Franzetti, A., & Vaccari, M. (2022). Remediation of soil polluted with petroleum hydrocarbons and its reuse for agriculture: Recent progress, challenges, and perspectives. Chemosphere, 293, 133572. https://doi.org/https://doi.org/10.1016/j.chemosphere.2022.133572
Bai, Y., Liang, H., Wang, L., Tang, T., Li, Y., Cheng, L., & Gao, D. (2023). Bioremediation of Diesel-Contaminated Soil by Fungal Solid-State Fermentation. Bulletin of Environmental Contamination and Toxicology, 112(1), 13. https://doi.org/10.1007/s00128-023-03840-3
Benguenab, A., & Chibani, A. (2021). Biodegradation of petroleum hydrocarbons by filamentous fungi (Aspergillus ustus and Purpureocillium lilacinum) isolated from used engine oil contaminated soil. Acta Ecologica Sinica, 41(5), 416-423. https://doi.org/https://doi.org/10.1016/j.chnaes.2020.10.008
Bennet, J., Wunch, K., & Faison, B. (2002). Use of fungi in biodegradation. Manual of environmental microbiology, 87, 960-971.
Blanc, D. C., Duarte, J. A., & Fiaux, S. B. (2024). Indigenous fungi with the ability to biodegrade hydrocarbons in diesel-contaminated soil are isolated and selected using a simple methodology. Environmental Pollution, 357, 124431. https://doi.org/https://doi.org/10.1016/j.envpol.2024.124431
Canul-Chan, M., Sánchez-González, M., González-Burgos, A., Zepeda, A., & Rojas-Herrera, R. (2018). Population structures shift during the biodegradation of crude and fuel oil by an indigenous consortium. International Journal of Environmental Science and Technology, 15(1), 1-16. https://doi.org/10.1007/s13762-017-1362-7
Cruz-Lachica, I., Marquez-Zequera, I., Allende-Molar, R., Sañudo-Barajas, J. A., Leon-Felix, J., Ley-Lopez, N., & Garcia-Estrada, R. S. (2018). Diversity of mucoralean fungi in soils of papaya (Carica papaya L.) producing regions in Mexico. Fungal Biology, 122(8), 810-816. https://doi.org/https://doi.org/10.1016/j.funbio.2018.04.008
García-Sánchez, M., Siles, J. A., Cajthaml, T., García-Romera, I., Tlustoš, P., & Száková, J. (2015). Effect of digestate and fly ash applications on soil functional properties and microbial communities. European Journal of Soil Biology, 71, 1-12. https://doi.org/https://doi.org/10.1016/j.ejsobi.2015.08.004
Gaur, S., Sahani, A., Chattopadhyay, P., Gupta, S., & Jain, A. (2023). Remediation of Waste Engine Oil Contaminated Soil using Rhamnolipid based Detergent Formulation. Materials Today: Proceedings, 77, 31-38. https://doi.org/https://doi.org/10.1016/j.matpr.2022.08.452
Gielnik, A., Pechaud, Y., Huguenot, D., Cébron, A., Riom, J.-M., Guibaud, G., Esposito, G., & van Hullebusch, E. D. (2019). Effect of digestate application on microbial respiration and bacterial communities' diversity during bioremediation of weathered petroleum hydrocarbons contaminated soils. Science of The Total Environment, 670, 271-281. https://doi.org/https://doi.org/10.1016/j.scitotenv.2019.03.176
Govarthanan, M., Fuzisawa, S., Hosogai, T., & Chang, Y.-C. (2017). Biodegradation of aliphatic and aromatic hydrocarbons using the filamentous fungus Penicillium sp. CHY-2 and characterization of its manganese peroxidase activity [10.1039/C6RA28687A]. RSC Advances, 7(34), 20716-20723. https://doi.org/10.1039/C6RA28687A
Houbron, E., Cruz-Carmona, E., Ponciano-Rosas, A., Rustrián-Portilla, E., & Canul-Chan, M. (2021). Motor oil wastewater treatment in a packed bed bioreactor using immobilized native microbial consortium. Revista Mexicana de Ingeniería Química, 20(2), 761-773.
Jacqueline, P. J., & Velvizhi, G. (2024). Substantial physicochemical pretreatment and rapid delignification of lignocellulosic banana, pineapple and papaya fruit peels: A study on physical-chemical characterization. Sustainable Chemistry and Pharmacy, 37, 101347. https://doi.org/https://doi.org/10.1016/j.scp.2023.101347
Koolivand, A., Abtahi, H., Parhamfar, M., Saeedi, R., Coulon, F., Kumar, V., Villaseñor, J., Sartaj, M., Najarian, N., Shahsavari, M., Seyedmoradi, P., Rahimi, L., & Bagheri, F. (2022). The effect of petroleum hydrocarbons concentration on competition between oil-degrading bacteria and indigenous compost microorganisms in petroleum sludge bioremediation. Environmental Technology & Innovation, 26, 102319. https://doi.org/https://doi.org/10.1016/j.eti.2022.102319
Li, Q., Liu, J., & Gadd, G. M. (2020). Fungal bioremediation of soil co-contaminated with petroleum hydrocarbons and toxic metals. Applied Microbiology and Biotechnology, 104(21), 8999-9008.
Lozano-Rodríguez, J. E. H.-R., A.C.; Sandoval-Elías, E.; González-López, G.; Canul-Chan, M.; Houbron, E. y Rustrián-Portilla, E. . (2018). Aislamiento De Hongos Con Potencial Para Degradar Hidrocarburos y Lignina. Revista del Centro de Graduados e Investigación. Instituto Tecnológico de Mérida, 33(73), 478-481. https://doi.org/https://www.revistadelcentrodegraduados.com/2019/11/aislamiento-de-hongos-con-potencial.html?m=0
Nwankwegu, A. S., Onwosi, C. O., Orji, M. U., Anaukwu, C. G., Okafor, U. C., Azi, F., & Martins, P. E. (2016). Reclamation of DPK hydrocarbon polluted agricultural soil using a selected bulking agent. Journal of Environmental Management, 172, 136-142. https://doi.org/https://doi.org/10.1016/j.jenvman.2016.02.032
Omoni, V. T., Bankole, P. O., Semple, K. T., Ojo, A. S., Ibeto, C., Okekporo, S. E., & Harrison, I. A. (2024). Enhanced Remediation of Polycyclic Aromatic Hydrocarbons in Soil Through Fungal Delignification Strategy and Organic Waste Amendment: A Review. Indian Journal of Microbiology. https://doi.org/10.1007/s12088-024-01383-7
Omoni, V. T., Lag-Brotons, A. J., & Semple, K. T. (2020). Impact of organic amendments on the development of 14C-phenanthrene catabolism in soil. International Biodeterioration & Biodegradation, 151, 104991.
Pimda, W., & Bunnag, S. (2015). Biodegradation of waste motor oil by Nostoc hatei strain TISTR 8405 in water containing heavy metals and nutrients as co-contaminants. Journal of Industrial and Engineering Chemistry, 28, 117-123. https://doi.org/https://doi.org/10.1016/j.jiec.2015.02.006
Puglisi, E., Cappa, F., Fragoulis, G., Trevisan, M., & Del Re, A. A. M. (2007). Bioavailability and degradation of phenanthrene in compost amended soils. Chemosphere, 67(3), 548-556. https://doi.org/https://doi.org/10.1016/j.chemosphere.2006.09.058
Rangel, D. E., Finlay, R. D., Hallsworth, J. E., Dadachova, E., & Gadd, G. M. (2018). Fungal strategies for dealing with environment-and agriculture-induced stresses. Fungal Biology, 122(6), 602-612.
Robichaud, K., Girard, C., Dagher, D., Stewart, K., Labrecque, M., Hijri, M., & Amyot, M. (2019). Local fungi, willow and municipal compost effectively remediate petroleum-contaminated soil in the Canadian North. Chemosphere, 220, 47-55. https://doi.org/https://doi.org/10.1016/j.chemosphere.2018.12.108
Sharma, A., Bachheti, A., Sharma, P., Bachheti, R. K., & Husen, A. (2020). Phytochemistry, pharmacological activities, nanoparticle fabrication, commercial products and waste utilization of Carica papaya L.: A comprehensive review. Current Research in Biotechnology, 2, 145-160. https://doi.org/https://doi.org/10.1016/j.crbiot.2020.11.001
Xiao, L., Zhao, X., Yao, J., Lu, Q., Feng, X., & Wu, S. (2024). Biodegradation and adsorption of benzo[a]pyrene by fungi-bacterial coculture. Ecotoxicology and Environmental Safety, 283, 116811. https://doi.org/https://doi.org/10.1016/j.ecoenv.2024.116811
Zain ul Arifeen, M., Ma, Y., Wu, T., Chu, C., Liu, X., Jiang, J., Li, D., Xue, Y.-R., & Liu, C.-H. (2022). Anaerobic biodegradation of polycyclic aromatic hydrocarbons (PAHs) by fungi isolated from anaerobic coal-associated sediments at 2.5 km below the seafloor. Chemosphere, 303, 135062. https://doi.org/https://doi.org/10.1016/j.chemosphere.2022.135062
Ahmad, R., Jilani, G., Arshad, M., Zahir, Z. A., & Khalid, A. (2007). Bio-conversion of organic wastes for their recycling in agriculture: an overview of perspectives and prospects. Annals of Microbiology, 57(4), 471-479. https://doi.org/10.1007/BF03175343
Al-Hawash, A. B., Zhang, J., Li, S., Liu, J., Ghalib, H. B., Zhang, X., & Ma, F. (2018). Biodegradation of n-hexadecane by Aspergillus sp. RFC-1 and its mechanism. Ecotoxicology and Environmental Safety, 164, 398-408.
Ambaye, T. G., Chebbi, A., Formicola, F., Prasad, S., Gomez, F. H., Franzetti, A., & Vaccari, M. (2022). Remediation of soil polluted with petroleum hydrocarbons and its reuse for agriculture: Recent progress, challenges, and perspectives. Chemosphere, 293, 133572. https://doi.org/https://doi.org/10.1016/j.chemosphere.2022.133572
Bai, Y., Liang, H., Wang, L., Tang, T., Li, Y., Cheng, L., & Gao, D. (2023). Bioremediation of Diesel-Contaminated Soil by Fungal Solid-State Fermentation. Bulletin of Environmental Contamination and Toxicology, 112(1), 13. https://doi.org/10.1007/s00128-023-03840-3
Benguenab, A., & Chibani, A. (2021). Biodegradation of petroleum hydrocarbons by filamentous fungi (Aspergillus ustus and Purpureocillium lilacinum) isolated from used engine oil contaminated soil. Acta Ecologica Sinica, 41(5), 416-423. https://doi.org/https://doi.org/10.1016/j.chnaes.2020.10.008
Bennet, J., Wunch, K., & Faison, B. (2002). Use of fungi in biodegradation. Manual of environmental microbiology, 87, 960-971.
Blanc, D. C., Duarte, J. A., & Fiaux, S. B. (2024). Indigenous fungi with the ability to biodegrade hydrocarbons in diesel-contaminated soil are isolated and selected using a simple methodology. Environmental Pollution, 357, 124431. https://doi.org/https://doi.org/10.1016/j.envpol.2024.124431
Canul-Chan, M., Sánchez-González, M., González-Burgos, A., Zepeda, A., & Rojas-Herrera, R. (2018). Population structures shift during the biodegradation of crude and fuel oil by an indigenous consortium. International Journal of Environmental Science and Technology, 15(1), 1-16. https://doi.org/10.1007/s13762-017-1362-7
Cruz-Lachica, I., Marquez-Zequera, I., Allende-Molar, R., Sañudo-Barajas, J. A., Leon-Felix, J., Ley-Lopez, N., & Garcia-Estrada, R. S. (2018). Diversity of mucoralean fungi in soils of papaya (Carica papaya L.) producing regions in Mexico. Fungal Biology, 122(8), 810-816. https://doi.org/https://doi.org/10.1016/j.funbio.2018.04.008
García-Sánchez, M., Siles, J. A., Cajthaml, T., García-Romera, I., Tlustoš, P., & Száková, J. (2015). Effect of digestate and fly ash applications on soil functional properties and microbial communities. European Journal of Soil Biology, 71, 1-12. https://doi.org/https://doi.org/10.1016/j.ejsobi.2015.08.004
Gaur, S., Sahani, A., Chattopadhyay, P., Gupta, S., & Jain, A. (2023). Remediation of Waste Engine Oil Contaminated Soil using Rhamnolipid based Detergent Formulation. Materials Today: Proceedings, 77, 31-38. https://doi.org/https://doi.org/10.1016/j.matpr.2022.08.452
Gielnik, A., Pechaud, Y., Huguenot, D., Cébron, A., Riom, J.-M., Guibaud, G., Esposito, G., & van Hullebusch, E. D. (2019). Effect of digestate application on microbial respiration and bacterial communities' diversity during bioremediation of weathered petroleum hydrocarbons contaminated soils. Science of The Total Environment, 670, 271-281. https://doi.org/https://doi.org/10.1016/j.scitotenv.2019.03.176
Govarthanan, M., Fuzisawa, S., Hosogai, T., & Chang, Y.-C. (2017). Biodegradation of aliphatic and aromatic hydrocarbons using the filamentous fungus Penicillium sp. CHY-2 and characterization of its manganese peroxidase activity [10.1039/C6RA28687A]. RSC Advances, 7(34), 20716-20723. https://doi.org/10.1039/C6RA28687A
Houbron, E., Cruz-Carmona, E., Ponciano-Rosas, A., Rustrián-Portilla, E., & Canul-Chan, M. (2021). Motor oil wastewater treatment in a packed bed bioreactor using immobilized native microbial consortium. Revista Mexicana de Ingeniería Química, 20(2), 761-773.
Jacqueline, P. J., & Velvizhi, G. (2024). Substantial physicochemical pretreatment and rapid delignification of lignocellulosic banana, pineapple and papaya fruit peels: A study on physical-chemical characterization. Sustainable Chemistry and Pharmacy, 37, 101347. https://doi.org/https://doi.org/10.1016/j.scp.2023.101347
Koolivand, A., Abtahi, H., Parhamfar, M., Saeedi, R., Coulon, F., Kumar, V., Villaseñor, J., Sartaj, M., Najarian, N., Shahsavari, M., Seyedmoradi, P., Rahimi, L., & Bagheri, F. (2022). The effect of petroleum hydrocarbons concentration on competition between oil-degrading bacteria and indigenous compost microorganisms in petroleum sludge bioremediation. Environmental Technology & Innovation, 26, 102319. https://doi.org/https://doi.org/10.1016/j.eti.2022.102319
Li, Q., Liu, J., & Gadd, G. M. (2020). Fungal bioremediation of soil co-contaminated with petroleum hydrocarbons and toxic metals. Applied Microbiology and Biotechnology, 104(21), 8999-9008.
Lozano-Rodríguez, J. E. H.-R., A.C.; Sandoval-Elías, E.; González-López, G.; Canul-Chan, M.; Houbron, E. y Rustrián-Portilla, E. . (2018). Aislamiento De Hongos Con Potencial Para Degradar Hidrocarburos y Lignina. Revista del Centro de Graduados e Investigación. Instituto Tecnológico de Mérida, 33(73), 478-481. https://doi.org/https://www.revistadelcentrodegraduados.com/2019/11/aislamiento-de-hongos-con-potencial.html?m=0
Nwankwegu, A. S., Onwosi, C. O., Orji, M. U., Anaukwu, C. G., Okafor, U. C., Azi, F., & Martins, P. E. (2016). Reclamation of DPK hydrocarbon polluted agricultural soil using a selected bulking agent. Journal of Environmental Management, 172, 136-142. https://doi.org/https://doi.org/10.1016/j.jenvman.2016.02.032
Omoni, V. T., Bankole, P. O., Semple, K. T., Ojo, A. S., Ibeto, C., Okekporo, S. E., & Harrison, I. A. (2024). Enhanced Remediation of Polycyclic Aromatic Hydrocarbons in Soil Through Fungal Delignification Strategy and Organic Waste Amendment: A Review. Indian Journal of Microbiology. https://doi.org/10.1007/s12088-024-01383-7
Omoni, V. T., Lag-Brotons, A. J., & Semple, K. T. (2020). Impact of organic amendments on the development of 14C-phenanthrene catabolism in soil. International Biodeterioration & Biodegradation, 151, 104991.
Pimda, W., & Bunnag, S. (2015). Biodegradation of waste motor oil by Nostoc hatei strain TISTR 8405 in water containing heavy metals and nutrients as co-contaminants. Journal of Industrial and Engineering Chemistry, 28, 117-123. https://doi.org/https://doi.org/10.1016/j.jiec.2015.02.006
Puglisi, E., Cappa, F., Fragoulis, G., Trevisan, M., & Del Re, A. A. M. (2007). Bioavailability and degradation of phenanthrene in compost amended soils. Chemosphere, 67(3), 548-556. https://doi.org/https://doi.org/10.1016/j.chemosphere.2006.09.058
Rangel, D. E., Finlay, R. D., Hallsworth, J. E., Dadachova, E., & Gadd, G. M. (2018). Fungal strategies for dealing with environment-and agriculture-induced stresses. Fungal Biology, 122(6), 602-612.
Robichaud, K., Girard, C., Dagher, D., Stewart, K., Labrecque, M., Hijri, M., & Amyot, M. (2019). Local fungi, willow and municipal compost effectively remediate petroleum-contaminated soil in the Canadian North. Chemosphere, 220, 47-55. https://doi.org/https://doi.org/10.1016/j.chemosphere.2018.12.108
Sharma, A., Bachheti, A., Sharma, P., Bachheti, R. K., & Husen, A. (2020). Phytochemistry, pharmacological activities, nanoparticle fabrication, commercial products and waste utilization of Carica papaya L.: A comprehensive review. Current Research in Biotechnology, 2, 145-160. https://doi.org/https://doi.org/10.1016/j.crbiot.2020.11.001
Xiao, L., Zhao, X., Yao, J., Lu, Q., Feng, X., & Wu, S. (2024). Biodegradation and adsorption of benzo[a]pyrene by fungi-bacterial coculture. Ecotoxicology and Environmental Safety, 283, 116811. https://doi.org/https://doi.org/10.1016/j.ecoenv.2024.116811
Zain ul Arifeen, M., Ma, Y., Wu, T., Chu, C., Liu, X., Jiang, J., Li, D., Xue, Y.-R., & Liu, C.-H. (2022). Anaerobic biodegradation of polycyclic aromatic hydrocarbons (PAHs) by fungi isolated from anaerobic coal-associated sediments at 2.5 km below the seafloor. Chemosphere, 303, 135062. https://doi.org/https://doi.org/10.1016/j.chemosphere.2022.135062
Publicado
2026-04-30
Sección
Artículos de Investigación
Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial 4.0.
Avisos de derechos de autor propuestos por Creative Commons
1. Política propuesta para revistas que ofrecen acceso abierto
Aquellos autores/as que tengan publicaciones con esta revista, aceptan los términos siguientes:
- Los autores/as conservarán sus derechos de autor y garantizarán a la revista el derecho de primera publicación de su obra, el cuál estará simultáneamente sujeto a la Licencia de reconocimiento de Creative Commons que permite a terceros compartir la obra siempre que se indique su autor y su primera publicación esta revista.
- Los autores/as podrán adoptar otros acuerdos de licencia no exclusiva de distribución de la versión de la obra publicada (p. ej.: depositarla en un archivo telemático institucional o publicarla en un volumen monográfico) siempre que se indique la publicación inicial en esta revista.
- Se permite y recomienda a los autores/as difundir su obra a través de Internet (p. ej.: en archivos telemáticos institucionales o en su página web) antes y durante el proceso de envío, lo cual puede producir intercambios interesantes y aumentar las citas de la obra publicada. (Véase El efecto del acceso abierto).
